BioTools

1 Kb ladder - 100 lanes (Vial 50 µg)

The 1 Kb Ladder is obtained from the digestion of different plasmids (pUC, phage, and sequences from yeast genome) by the corresponding restriction enzymes.

1 Kb ladder Ready to Use - 100 lanes (Vial 50 µg)

The 1 kb DNA Ladder is prepared from vector DNA digested to completion with appropriate restriction enzymes to yield bands ranging from 250 bp to 10 kb, suitable for use as molecular weight standards for agarose gel electrophoresis.

100 bp ladder - 100 lanes (Vial 50 µg)

The 100 bp Ladder is obtained from the digestion of a customised plasmid (pUC, phage, and sequences from yeast genome) by the corresponding restriction enzymes.

100 bp ladder Ready to Use - 100 lanes (Vial 50 µg)

The 100 bp DNA Ladder is prepared from plasmid DNA digested to completion with appropriate restriction enzymes to yield bands ranging from 100 bp to 1 kb, suitable for use as molecular weight standards for agarose and polyacrylamide gels.

BIOFOOD MIXED kit (homogeneous samples) (48 rxns)

Biofood Mixed kit has been designed to detect and identify six different animal species commonly used in food and feed samples (cow, pork, chicken, horse, goat and sheep) through the amplification of the cytochrome b gene, a highly conserved mitochondrial gene.

BIOFOOD STANDARD kit - Detection of animal species in food (48 rxns)

Biofood Standard kit is a multiplex PCR reaction designed to detect the presence of vertebrate and plant material in food samples.

BIOGENICS Bt-11 MAIZE ID kit (48 rxns)

Biogenics Bt-11TM Maize ID kit has been designed to detect Bt-11TM Maize, in fresh and processed samples.

BIOGENICS Bt-176 MAIZE ID kit (48 rxns)

BIOGENICS Bt-176 MAIZE kit is based on the detection and amplification of gene regions specific for Bt-176 maize (cry1Ab gene from Bacillus thurigiensis) and not present in native maize, as well as GMO generic sequences (35S promoter ), present in approximately 90 % of the GMOs that have been commercialised up to date.

BIOGENICS MON810 MAIZE ID kit (48 rxns)

Biogenics MON810TM Maize ID kit has been designed to detect MON810TM Maize, in fresh and processed samples.

BIOGENICS RoundUp Ready® Soya ID kit (48 rxns)

Biogenics RoundUpTM Ready Soya QT kit has been designed for the detection of RoudUpTM Soya in fresh and processed food by Real Time PCR.

BIOGENICS STD kit - General detection of GMOs in food (48 rxns)

Biogenics Standard kit has been designed to detect generic GMOs in fresh and processed samples.

BIOGENICS T25 MAIZE ID kit (48 rxns)

Biogenics T25 Maize ID kit has been designed to detect T25 Maize, in fresh and processed samples.

BIOHBV kit (96 rxns)

BIOHBV kit has been designed to detect hepatitis B virus in a wide variety of clinical samples (e.g. blood, serum, plasma, PBMCs, hepatic tissues).

BIOMALAR Gel Form kit

The Biomalar Gel Form kit allows the qualitative detection of Plasmodium species in clinical samples.

BIOPAP kit (96 rxns)

BIOPAP kit has been designed for detection of human papillomavirus (HPV) in clinical samples and differentiation between oncogenic and non-oncogenic genotypes, in a single reaction.

BIOTOOLS 10x Certamp Complex Buffer MgCl2 FREE - 250 rxns

Certamp Complex Buffer is a 10X Reaction buffer [75 mM Tris HCl (pH 9.1), (NH4)2SO4, Tween 20, Glycerol, cofactors and enhancers] supplied with Certamp kit for Complex Amplification.

BIOTOOLS 10x Certamp Long Buffer MgCl2 FREE - 250 rxns

Certamp Long Buffer is a 10X Reaction buffer [75 mM Tris HCl (pH 9.1), (NH4)2SO4, Tween 20, Glycerol] supplied with Certamp kit for Long Amplification.

BIOTOOLS 10x Reaction Buffer MgCl2 FREE - 1,8ml

Reaction Buffer MgCl2 Free is a 10X Reaction buffer [75 mM Tris HCl (pH 9.0), 50 mM KCl, 20 mM (NH4)2SO4]

BIOTOOLS 10x Standard Reaction Buffer with MgCl2 - 1,8ml

Standard Reaction Buffer is a 10X Reaction buffer [75 mM Tris HCl (pH 9.0), 2 mM MgCl2, 50 mM KCl, 20 mM (NH4)2SO4]

BIOTOOLS 50mM MgCl2 solution - 1,8ml

Magnesium chloride solution is provided with Biotools DNA Polymerases for optimising the magnesium concentration.

BIOTOOLS dNTPs mix (10 mM each)

dNTP Mix is designed to facilitate routine laboratory work.

Biotools High Retrotranscriptase

Biotools High Retrotranscriptase is a thermostable, new recombinant reverse transcriptase expressed in E. Coli.

Biotools High Retrotranscriptase - Starter Kit

Biotools High Retrotranscriptase-Starter Kit is a complete system for efficient synthesis of first strand cDNA.

BIOTOOLS HR Agarose (high resolution electrophoresis)

HR Agarose is specifically designed for high resolution electrophoresis of DNA and RNA fragments.

BIOTOOLS MB Agarose

MB Agarose resolves DNA and RNA fragments from 0.2 to 22 kb.

BIOTOOLS Mineral Oil - 2ml

BIOTOOLS Ultrapure Water - 5ml

BIOTUB kit (96 rxns)

BIOTUB kit has been designed to detect human Mycobacterium tuberculosis by DNA amplification, in clinical samples (blood, lung tissue and sputum).

CERTAMP Kit for COMPLEX AMPLIFICATIONS

Certamp kits include an exclusive combination of proof-reading and non-proofreading DNA Polymerases, together with cofactors and an enhancing buffer to increase the overall yield of long and/or difficult amplification reactions.

CERTAMP Kit for LONG AMPLIFICATIONS

Certamp kits for Long Amplifications include an exclusive combination of proof-reading and non-proofreading DNA Polymerases, together with cofactors and an enhancing buffer to increase the overall yield of long and/or difficult amplification reactions.

DNA AmpliGel Master Mix Strips

The process is simple because all the reagents needed for the PCR reaction are included in each vial, so just add the sample (DNA and primers).

DNA AmpliGel Master Mix-Plates

Biotools has developed a new PCR product using one of its international patents: GELIFICATION.

DNA AmpliGel PLUS Master Mix Strips

This enzyme is a recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5'-3' polymerase.

DNA Amplitools Complex Master Mix

The DNA AmpliTools Complex Master Mix from Biotools is a 2X master mix optimised to achieve maximum yield and specificity in amplification reactions using complex (genomic DNA, GC-rich templates, templates with secondary structures) and/or scarce DNA templates.

DNA Amplitools Fast Master Mix

The new developed DNA AmpliTools Fast Master Mix (2X) is a sensitive, premix solution for performing rapid cycling protocols of a wide range of templates (including genomic DNAs and templates rich in CG-content).

DNA AmpliTools Green Master Mix

The new developed DNA AmpliTools Green Master Mix (2X) is a ready-to-use solution for performing PCR amplifications of a wide range of templates (including genomic DNAs and templates rich in CG-content).

DNA Amplitools HotSplit Master Mix

The new developed DNA AmpliTools HotSplit Master Mix (2X) is an optimised ready-to-use solution containing HotSplit DNA Polymerase for performing PCR amplifications of a wide range of templates (including genomic DNAs and templates rich in CG-content).

DNA Amplitools Master Mix

The new developed DNA AmpliTools Master Mix (2X) is a ready-to-use solution for performing PCR amplifications of a wide range of templates (including genomic DNAs and templates rich in CG-content).

DNA AmpliTools Multiplex Master Mix

The new developed DNA AmpliTools Multiplex Master Mix (2X) is a sensitive, premix solution for performing simultaneous amplification of multiple targets using multiple primers.

DNA Controls

DNAs from different animal species are available to use as quality controls in the Agfood Line kits.

DNA Polymerase 1U/µl

Recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5'-3' polymerase and 5'-3' exonuclease activities and a reduced error rate (1 - 10 x 10-6).

DNA Polymerase 5U/µl

Recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5'-3' polymerase and 5'-3' exonuclease activities and a reduced error rate (1 - 10 x 10-6).

dNTPs mix (25 mM each)

dNTP Mix is designed to facilitate routine laboratory work.

FiX174/Hinfl - 100 lanes (Vial 50 µg)

The FiX174 DNA is completely digested by the HinfI enzyme.

HIGH RNase INHIBITOR

High RNase Inhibitor inhibits, by binding in a potent non-competitive inhibitor of a wide spectrum of RNases, including RNase A, RNase B and RNase C.

High Scriptools OneStep Kit

High Scriptools OneStep Kit is a novel RT-PCR system for the detection of specific sequences within an RNA sample.

High ScripTools-QUANTIMIX Easy Kit

High Scriptools-Quantimix Easy Kit is a novel real-time RT-PCR system for the quantification of specific sequences within an RNA sample.

High Scriptools-Quantimix Easy Master Mix (High SQE Master Mix)

High Scriptools-Quantimix Easy Master Mix (High SQE Master Mix) is a novel RT-PCR system for the detection of specific sequences within an RNA sample.

High Scriptools-Quantimix Easy Probes Kit

High Scriptools-Quantimix Easy Probes Kit is a novel real-time RT-PCR system for the quantification of RNA targets in an easy-to-handle format.

HotSplit DNA Polymerase 1U/µl

Biotools HotSplit DNA Polymerase is ideal for hot start amplification of DNA fragments with improved specificity over our conventional DNA Polymerase.

HotSplit DNA Polymerase 5U/µl

Biotools HotSplit DNA Polymerase is ideal for hot start amplification of DNA fragments with improved specificity over our conventional DNA Polymerase.

HUMAN REFERENCE GENE PANEL

Los Human Reference Gene Panel (TATAA Biocenter) consisten en 12 ensayos de qPCR validados para los genes de control endógeno más comunes.

Human Single Assays

Human Genes Individual Controls (TATAA Biocenter) proporciona una manera rápida y rentable de identificar genes de control en ensayos de qPCR.

Individual dNTPs

10 mM dNPTs, dATP, dCTP, dGTP, and dTTP (2'-desxynucleoside'triphosphates) have been designed to substitute the dNTP mixes in experiments using each of them independently (e.g. reactions using labelled nucleotides).

Lambda/HindIII - 100 lanes (Vial 50 µg)

Lamda DNA is completely digested by the HindIII enzyme.

LEVATOOLS DNA Polymerase 5U/µl

Levatools DNA Polymerase is a DNA Polymerase cloned and purified in Saccharomyces cerevisiae yeast.

Master Mix (High SQEP Master Mix)

High Scriptools-Quantimix Easy Probes Master Mix (High SQEP Master Mix) is a novel real-time RT-PCR system for the quantification of RNA targets designed to be used with hydrolysis probes (e.g. TaqMan probes).

MOUSE ENDOGENOUS CONTROL GENE PANEL

Los Mouse Reference Gene Panel (TATAA Biocenter) consisten en 12 ensayos de qPCR validados para los genes de control endógeno más comunes (ver Tabla)

MYCOPLASMA Gel Form kit

The Mycoplasma Gel Detection kit from Biotools allows Mycoplasma contamination in cell culture supernatants to be detected by endpoint PCR by amplification of a conserved region of 16S rRNA.

Oligo dT(15) - 30ug

Oligo dT(15) primer is designed to initiate synthesis of a cDNA from total RNA in a reverse transcription reaction as well as for its use in generating labeled cDNA for screening microarrays.

pBR322/BsuRI - 100 lanes (Vial 50 µg)

The plasmid pBR 322 is completely digested by the BsuRI enzyme.

Pfu DNA polymerase 1U/µl

High-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3'-5' exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases).

Pfu DNA polymerase 5U/µl

High-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3'-5' exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases).

QUANTIMIX EASY kit

QUANTIMIX EASY KIT has been optimised to deliver maximum efficiency, precision, and sensitivity during nucleic acid amplification in real time using intercalating fluorophores.

QUANTIMIX EASY PROBES kit

Biotools Quantimix Easy Probes kit allows the nucleic acid amplification by Real Time using hydrolysis and hybridization probes.

QUANTIMIX HotSplit Easy kit

QUANTIMIX HOTSPLIT EASY KIT has been optimised to deliver maximum efficiency, precision, and sensitivity during nucleic acid amplification in real time using intercalating fluorophores.

QUANTIMIX HotSplit Probes kit

QUANTIMIX HOTSPLIT PROBES KIT is a master mix optimized for real-time amplification tests using fluorescent hydrolysis probes for the detection and quantification of template DNA.

Random Primers - 30ug

Random Primers is a combination of single-stranded random hexanucleotides with 5'- and 3'- hydroxyl ends.

Rat/Mouse Single Assays

Los Mouse Reference Gene Panel (TATAA Biocenter) consisten en 12 ensayos de qPCR validados para los genes de control endógeno más comunes.

SPEEDTOOLS DNA Extraction kit

SpeedTools DNA extraction kit is faster and easier than traditional protocols which use density gradients and/or hazardous organic solvents.

SPEEDTOOLS FOOD DNA Extraction Kit

SpeedTools Food DNA Extraction kit guarantees a good recovery of small DNA fragments (<1 kb) from processed or complex food matrices.

SPEEDTOOLS PCR Clean-Up kit

SpeedTools PCR Clean-up kit offers a rapid and easy way to purify amplified DNA fragments directly from PCR reactions or from agarose gels (TAE/TBE).

SPEEDTOOLS PLANT DNA Extraction kit

The SpeedTools Plant DNA Extraction kit technology combines the advantages of a highly efficient lysis buffer based on the CTAB procedure, with a powerful novel method for nucleic acid isolation and purification characterised by the use of non-chaotropic salts for the binding of nucleic acids to a solid phase.

SPEEDTOOLS Plasmid DNA Purification kit

SpeedTools Plasmid DNA Purification kit is designed for a rapid and easy preparation of up to 20 µg of highly pure plasmid DNA from 0.5 to 2 ml bacterial suspension (mini preps).

SPEEDTOOLS RNA Virus Extraction kit

SpeedTools RNA-Virus Extraction kit, viral nucleic acids (e.g. HCV, HIV, CMV) can be purified from:Serum, Urine, Plasma, Bronchoalveolar lavage fluid, Other body fluids

SPEEDTOOLS TISSUE DNA Extraction kit

SpeedTools Tissue DNA extraction kit is perfect for preparing total DNA (genomic and mitochondrial) from a wide variety of samples (see below).

SPEEDTOOLS Total RNA Extraction kit

SpeedTools Total RNA Extraction kit is a powerful kit recommended for the isolation of total RNA from: Cultured cells, yeast, cell-free biological fluids bacteria, paraffin embedded tissue tissues.

ULTRATOOLS DNA Polymerase 1U/µl

Ultratools DNA Polymerase is a recombinant DNA Polymerase from Thermus s.p. and cloned in E. coli, which undergoes an additional nonchromatographic method of purification.

ULTRATOOLS DNA Polymerase 5U/µl

Ultratools DNA polymerase is a recombinant DNA Polymerase from Thermus s.p. and cloned in E. coli, which undergoes an additional nonchromatographic method of purification.