pCSG-IBA43 vector

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Mammalian expression vector encoding an N-terminal His-tag and C-terminal Strep-tag®II
pCSG-IBA43 vector

pCSG-IBA43 is a large expression vector with universal features for transient expression of target proteins with an N-terminal His-tag and C-terminal Strep-tag®II as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells. Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

  • Affinity Tag C'-terminal: Strep-tag®II
  • Affinity Tag N'-terminal: 6xHis
  • Cloning Method: Direct cloning using restriction enzyme Esp3I
  • Concentration: 250 ng/µl
  • Expression Host: Mammalian cells
  • Form: Suspension in TE buffer
  • Possible Application: Vector for recombinant expression in mammalian cells
  • Promoter: CMV Promoter
  • Resistance: Ampicillin, Neomycin
  • Sequence: See Documents
  • Size: 10926 bp

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