pENTRY-IBA51 vector
IBA Lifescience Kontakt z doradcąEntry vector for generation of StarGate donor vectors
StarGate® Entry Vector is designed to insert the PCR product of a certain gene of interest (GOI) to generate a Donor Vector. StarGate cloning via the pENTRY-IBA vector provides a tool for the systematic and rapid screen of the best working expression conditions for a certain GOI, where nothing is known from literature. After initial pENTRY cloning, the GOI in the resulting Donor Vector can be easily transferred by a simple one-tube reaction into a multitude of expression vectors (Acceptor Vectors) providing different features.
Specifications
- Cloning Method: Direct cloning using restriction enzyme Esp3I
- Concentration: 250 ng/µl
- Expression Host: E. coli
- Form: Suspension in TE buffer
- Promoter: tet Promoter
- Resistance: Kanamycin
- Sequence: See Documents
- Size: 2556 bp
