pESG-IBA164 vector

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Mammalian expression vector encoding an N-terminal Twin-Strep-tag® and C-terminal FLAG-tag
pESG-IBA164 vector

The pESG-164 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with an N-terminal Twin-Strep-tag® and C-terminal FLAG-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

  • Affinity Tag C'-terminal: FLAG
  • Affinity Tag N'-terminal: Twin-Strep-tag®
  • Cloning Method: Direct cloning using restriction enzyme Esp3I
  • Concentration: 250 ng/µl
  • Expression Host: Mammalian cells
  • Form: Suspension in TE buffer
  • Possible Application: Vector for recombinant expression in mammalian cells
  • Promoter: CMV Promoter
  • Resistance: Ampicillin, Neomycin
  • Sequence: See Documents
  • Size: 6190 bp

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