pLSG-IBA142 vector

IBA Lifescience Kontakt z doradcą
Insect expression vector for secretion of N-terminal His-tag and C-terminal Twin-Strep-tag® fusion proteins
pLSG-IBA142 vector

The pLSG-IBA142 vector is designed for high-level expression of target proteins with an N-terminal His-tag and C-terminal Twin-Strep-tag® in insect cells. The gene transfer into the polyhedrin gene locus of AcMNPV DNA is achieved by homologous recombination and the vector carries a polyhedrin promoter as well as the BM40 signal for secretion of the recombinant protein. The Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or with circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild-type virus to great extent. For selection and propagation in E. coli, the vector carries an ampicillin resistance and ColE1 origin of replication (pUC). Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

  • Affinity Tag C'-terminal: Twin-Strep-tag®
  • Affinity Tag N'-terminal: 6xHis
  • Cloning Method: Direct cloning using restriction enzyme Esp3I
  • Concentration: 250 ng/µl
  • Expression Host: Insect cells
  • Form: Suspension in TE buffer
  • Possible Application: Vector for recombinant expression in insect cells
  • Promoter: Polyhedrin Promoter
  • Resistance: Ampicillin
  • Sequence: See Documents
  • Size: 6326 bp

Podobne produkty

MEXi-293E cells

MEXi-293E cells

IBA Lifescience
Więcej
Biotin

Biotin

IBA Lifescience
Więcej
Strep-Tactin®XT 4Flow® high capacity cartridge

Strep-Tactin®XT 4Flow® high capacity cartridge

IBA Lifescience
Więcej