pYSG-IBA23 vector

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Yeast expression vector encoding an N-terminal GST-tag and C-terminal Strep-tag®II
pYSG-IBA23 vector

The pYSG-IBA23 vector is designed for high-level expression of target proteins with an N-terminal GST-tag and C-terminal Strep-tag®II in yeast. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, the ColE1 origin for a high plasmid copy number, the copper-inducible promoter (CUP1) for controlled high-level expression, the URA3 auxotrophy marker for selection after transformation (do not use URA3 for selection during expression), the LEU2d auxotrophy marker for selection to increase plasmid copy number for expression (do not use LEU2d for selection after transformation), and the 2 micron ori for episomal replication in yeast. Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

  • Affinity Tag C'-terminal: Strep-tag®II
  • Affinity Tag N'-terminal: GST (removal via PreScission® Protease site)
  • Cloning Method: Direct cloning using restriction enzyme Esp3I
  • Concentration: 250 ng/µl
  • Expression Host: Yeast
  • Form: Suspension in TE buffer
  • Possible Application: Vector for recombinant expression in yeast
  • Promoter: CUP1 Promoter
  • Resistance: Ampicillin
  • Sequence: See Documents
  • Size: 8540 bp

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