10x Buffer E

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Strep-Tactin® elution buffer with desthiobiotin
10x Buffer E

10x Buffer E is a 10x concentrated Strep-Tactin® elution buffer. The contained desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin® in a competitive manner, resulting in a mild elution. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin®XT resins.

Specifications

  • Concentration: 10x concentrated
  • Form: Solution
  • Possible Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins

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