Protein A Agarose columnIBA Lifescience Kontakt z doradcą
Protein A is commonly used for the affinity purification of monoclonal and polyclonal IgG class antibodies using affinity chromatography. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region and purifies all antibodies of the target class without regard to antigen specificity. Antibodies from serum (polyclonal antibodies), ascites, or cell culture supernatant of hybridoma cell lines (monoclonal antibodies) can thus be easily isolated.
The Protein A Agarose Gravity Flow column has a high dynamic binding capacity combined with an excellent NaOH stability.
- Bead Size: 45 µm
- Dynamic Protein Binding Capacity: ≥70 mg/ml resin
- Form: Pre-packed column
- Possible Application: Purification of monoclonal and polyclonal antibodies
- Specificity: Fc-region of IgG antibodies
- Support: Rigid, highly crosslinked agarose