Strep-Tactin® Sepharose® column

Strep-Tactin® Sepharose® columns contains 4% agarose coupled with the streptavidin variant Strep-Tactin® and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. Due to the low concentrated agarose, it is particularly suitable for large proteins, especially if the affinity to Strep-Tactin®XT 4Flow® is too high. The wide-meshed polymer allows for the simple attachment of larger proteins to the stationary phase.

To reduce the waiting period during sample and buffer application, the use of the application aid WET FRED is recommended.