Strep-Tactin® Sepharose® resin

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50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Strep-Tactin® Sepharose® is a 4% agarose coupled with the streptavidin variant Strep-Tactin® and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. Due to the low concentrated agarose, it is particularly suitable for large proteins, especially if the affinity to Strep-Tactin®XT 4Flow® is too high. The wide-meshed polymer allows for the simple attachment of larger proteins to the stationary phase. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension is necessary. Please note that Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to the low pressure stability.

Specifications

  • Bead Size: 45 - 165 µm
  • Biotin Binding Capacity: > 300 nmol/ml resin
  • Exclusion Limit: 3 x 10^7
  • Form: 50% suspension
  • Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
  • Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin
  • Strep-tag®II Binding Capacity: 3 mg/ml
  • Support: 4% agarose

Videos

Packing of a Gravity Flow Column for Protein Purification

Strep-Tactin® Sepharose® Color based functionality check

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