Strep-Tactin® Superflow® high capacity resin

IBA Lifescience Kontakt z doradcą
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Strep-Tactin® Superflow® high capacity is a 6% agarose coupled with the streptavidin variant Strep-Tactin® and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. In comparison to Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity has a higher density of immobilized Strep-Tactin®, which leads to a 3- to 5-fold higher yield of purified protein.

The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension is necessary.

Specifications

  • Bead Size: 60 - 160 µm spherical
  • Biotin Binding Capacity: > 900 nmol/ml resin
  • Dynamic Protein Binding Capacity: 7 mg/ml resin
  • Exclusion Limit: 6 x 10^6
  • Form: 50% suspension
  • Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
  • Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin
  • Support: 6% agarose, crosslinked

Videos

Packing of a Gravity Flow Column for Protein Purification

Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared

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