Strep-Tactin® Superflow® resin

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50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin

Strep-Tactin® Superflow® is a 6% agarose coupled with the streptavidin variant Strep-Tactin® and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins.

The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension is necessary.

Specifications

  • Bead Size: 60 - 160 µm spherical
  • Biotin Binding Capacity: > 300 nmol/ml resin
  • Exclusion Limit: 6 x 10^6
  • Form: 50% suspension
  • Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
  • Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin
  • Strep-tag®II Binding Capacity: 3 mg/ml
  • Support: 6% agarose, crosslinked

Videos

Packing of a Gravity Flow Column for Protein Purification

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