Strep-Tactin®XT 4Flow® resin

Strep-Tactin®XT 4Flow® is a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-Tactin®XT 4Flow® enables the application of denaturing agents such as urea.

The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.