CD81 Fab-TACS® Exosome Agarose Column Starter Kit, human

Fab-TACS® is a traceless affinity exosome isolation approach based on our Strep-tag® technology. CD81 Fab-TACS® Exosome Agarose Column Starter Kit allows label-free isolation of human CD81+ exosomes from plasma, serum and cell culture supernatants. CD81 is a member of the tetraspanin family present on the surface of exosomes. Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Exosomes adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target particles are efficiently washed away. In a final step, the addition of biotin causes the elution of the exosomes and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the exosome surface due to their low affinity. The label-free, fully functional exosomes are now ready for further downstream applications.

Advantages:

• Highly pure exosomes
• Fully functional exosomes
• Reversible binding to the target
• Label-free exosomes due to low affinity Fab fragments
• Quick and simple protocol without ultracentrifugation

The kit contains:

• Strep-Tactin® TACS Agarose columns, 0.3 ml (2x)
• CD81 Fab-Strep, human
• Biotin stock solution
• TACS Column Adapter