Zeocin™ | Selection antibiotic: endotoxin tested, sterile reagent
InvivoGen is the sole worldwide producer of Zeocin™.
Zeocin™ is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it.
The action of Zeocin™ is effective on most aerobic cells. Therefore, Zeocin™ is a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).
Resistance to Zeocin™ is conferred by the Sh ble gene product from Streptoalloteichus hindustanus, which inactivates Zeocin™ upon binding to the antibiotic [1-3].
Sh ble is a small 370bp-sized gene and is carried in a number of InvivoGen’s vectors.
Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.
For research use only
References:
- Drocourt D. et al., 1990. Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009.
- Gatignol A. et al., 1988. FEBS Letters. 230: 171-5.
- Dumas P. et al., 1994. Embo J. 242 (5) 595-601.
