QUANTI-Blue™ Solution is a colorimetric enzyme assay developed to determine any alkaline phosphatase (AP) activity in a biological sample, such as cell culture supernatant. It has been optimized for use in 96-well plates (standard procedure) and in 1536-well plates (high-throughput screening procedure).
QUANTI-Blue™ Solution can be used to detect and quantify AP or SEAP activity. Secreted embryonic alkaline phosphatase (SEAP), a truncated form of placental AP, is a widely used reporter gene. SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters. InvivoGen provides a wide collection of reporter cell lines with the SEAP reporter gene.
Features and advantages of QUANTI-Blue™ Solution:
- A convenient, easy and rapid means to detect and quantify SEAP and AP activity
- Short hands-on-time: < 10 min
- Easy to prepare: just mix QB Reagent and QB Buffer with water
- Highly sensitive
- Wide dynamic range High saturation threshold
- Allows for high-throughput screening (HTS)
In the presence of AP or SEAP, the color of QUANTI-Blue™ changes from pink to purple/blue. The purple/blue color intensity reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.
