Primocin™

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Antimicrobial agent for primary cells
Primocin™

Primocin™ is designed to offer complete protection of primary cells from microbial contamination. Primocin™ is a broad-spectrum antibiotic formulation that is gentle on your cells but lethal to the microbes. There are a number of common sources of microbial contamination especially in the isolation of primary cells from both animal and human tissue, including commensal flora and/or subclinical infections.

Key Features:

  • Active against bacteria, mycoplasma, and fungi
  • A common addition to various standardized primary cell culture protocols
  • Used throughout culturing process (e.g. culture media, wash solutions, and biopsy storage media)
  • Highly cited in the literature

Primocin™ is composed of four compounds, of which three, act directly on Gram-positive bacteria, Gram-negative bacteria, and mycoplasmas. The primary mode of action for these compounds is to block microbial DNA and protein synthesis. The fourth compound in Primocin™ eradicates fungi, including yeasts, by directly targeting processes at the fungal membrane.

Primocin™ is successfully used in many types of primary cell cultures (see citations) including:

  • Differentiated mouse- and human-derived cells such as astrocytes and immune cells (e.g. natural killer cells)
  • Stem cells such as mouse- and human-derived embryonic and pluripotent stem cells
  • 3D cellular models (i.e. organoid and spheroids) such as colon epithelial and carcinoma organoids

Primocin™ is made in-house at InvivoGen and is of the highest quality with rigorous quality control performed to ensure the absence of lot-to-lot variation.

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