Poly(dA:dT) Rhodamine

Poly(dA:dT) Rhodamine was chemically labeled by covalent coupling of a rhodamine probe containing a reactive alkylating group. This confers fluorescent properties to Poly(dA:dT) with a slight reduction of pattern recognition receptor (PRR) stimulatory activity.

Poly(dA:dT) is a poly(deoxyadenylic-deoxythymidylic) acid sodium salt and a repetitive double-stranded DNA (dsDNA) sequence of poly(dA-dT)•poly(dT-dA). Poly(dA:dT) is a synthetic analog of B-DNA.

Intracellular poly(dA:dT) is recognized by several cytosolic DNA sensors, triggering an immune response.

Furthermore, poly(dA:dT) is indirectly sensed by RIG-I leading to type I IFN production.