E. coli OMVs

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Caspase 11-4/5 inducer; E. coli outer membrane vesicles (OMVs)
E. coli OMVs

Outer membrane vesicles (OMVs) are small, spherical bodies secreted by Gram-negative bacteria, recently described as potent caspase 11-4/5 inflammasome inducers. They contain various pathogen-associated molecular patterns (PAMPs) such as lipoproteins and lipopolysaccharides (LPS; also known as endotoxin). These PAMPs initiate a myriad of immune responses through cell-surface receptors such as Toll-like receptors (TLRs) [1] as well as intracellular receptors, which leads to the formation of inflammasomes [2].

InvivoGen's E. coli OMVs have been purified from Escherichia coli BL21 using an optimized procedure, importantly providing lot-to-lot reproducibility.

Each batch of E. coli OMVs is thoroughly tested to characterize the OMV size and to ensure the activation of both TLR2 and TLR4 by the presence of lipoproteins and LPS, respectively.

Importantly, activation of the caspase 11-4/5 non-canonical inflammasome is confirmed using THP1-HMGB1::Lucia™ cells, measuring the release of the alarmin HMGB1 due to inflammasome-mediated cell death.

InvivoGen provides two grades of E. coli OMVs:

  • E. coli OMVs (standard preparation) - recommended for in vitro studies
  • E. coli OMVs InvivoFit™ - recommended for in vivo studies.

Specifically, E. coli OMVs InvivoFit™ are guaranteed sterile and have an endotoxin level >105 EU/mg of OMVs.

References:

  1. Kaparakis-Liaskos, M. & Ferrero, R.L., 2015. Immune modulation by bacterial outer membrane vesicles. Nat Rev Immunol. 15:375-387.
  2. Russo, A.J. et al, 2018. Emerging insights into noncanonical inflammasome recognition of microbes. J Mol Biol. 430:207-216.

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