Anti-hCD14-IgG is a chimeric monoclonal antibody specific for human CD14. It has been selected for its ability to efficiently neutralize the biological activity of TLR2 or TLR4.
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that acts as a bacterial pattern recognition receptor [1]. It is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2]. It binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections [3].
Anti-hCD14-IgG was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography.
References:
- Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.
- Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129-35.
- Aderem A. & Ulevitch R.J. , 2000. Toll-like receptors in the induction of the innate immune response. Nature. 406(6797):782-7.
