Anti-hCD14-IgG is a chimeric monoclonal antibody specific for human CD14. It has been selected for its ability to efficiently neutralize the biological activity of TLR2 or TLR4.
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that acts as a bacterial pattern recognition receptor . It is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 . It binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections .
Anti-hCD14-IgG was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography.
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