Anti-Flagellin FliC is a mouse monoclonal antibody to Salmonella typhimurium flagellin. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species.
Flagellin is recognized by TLR5 [1] and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex [2].
This MAb was generated by immunizing by DNA vaccination Balb/c mice with the fliC gene and screened by ELISA using purified flagellin (FLA-ST). The antibody was purified by Protein G affinity chromatography.
References:
- Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
- Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.
