LumiKine™ Xpress hIFN-β 2.0 is InvivoGen’s second generation bioluminescent human interferon-beta (hIFN-β) ELISA, using a Lucia luciferase-conjugated detection antibody. LumiKine™ Xpress hIFN-β 2.0 is an optimized ‘sandwich’ ELISA kit designed to rapidly and reliably measure the concentration of hIFN-β in various biological samples (cell culture supernatant, serum and plasma).
After a single 2-hour incubation, the detection of hIFN-β is instantaneously determined using QUANTI-Luc™ Plus, a Lucia luciferase detection reagent. The bioluminescent signal is readily assessed by luminometry.
Features of LumiKine™ Xpress hIFN-β 2.0:
- Single incubation: 2-hours
- Limit of Detection: 10 pg/ml
- Specificity: Human IFN-β
- Optimized antibody pairing: The capture and detection antibodies bind to different epitopes of the cytokine
- No cross-reactivity with hIFN-α2, murine (m)IFN-α2 or mIFN-β
LumiKine™ Xpress hIFN-β 2.0 is provided with a standard recombinant hIFN-β produced in mammalian cells, to ensure the most accurate and sensitive calibration for the measurement of unknown levels of hIFN-β.