CR3022-derived Anti-SARS-CoV-2 RBD mAbsInvivoGen Kontakt z doradcą
InvivoGen provides a family of Anti-Spike RBD CR3022-derived monoclonal antibodies (mAbs) in multiple human and mouse isotypes. These mAbs feature:
- the variable region of the CR3022 human mAb that specifically targets the Spike receptor-binding domain (RBD) 
- a constant region that mediates different effector functions depending on the isotype (human IgG1, hIgG1NQ, hIgA1, or hIgM, and mouse IgG2a or mIgG1e3 ) (See Table)
The Anti-Spike RBD clone CR3022 is a SARS-CoV neutralizing antibody that was obtained from the screening of an antibody-phage library and converted to a human IgG1 format . SARS-CoV and SARS-CoV-2 (2019-nCoV) RBD share a highly conserved epitope (86% shared identity) that is recognized by CR3022 [2, 3]. To date, the neutralization potency of CR3022 for SARS-CoV-2 is still unclear [3, 4]. Indeed, CR3022 does not interfere with the ACE2 binding motif [2-4].
- Detection of the presence of viral particles in culture supernatant or in serum (ELISA)
- Control of antibody-mediated effector functions (i.e. absence, increase or decrease in ADCC, ADCP, or CDC) (See Table)
- Isotype confirmed by ELISA
- Functionality validated by ELISA using a coated Spike-RBD-His fusion peptide
Our CR3022 variants have been generated by recombinant DNA technology, produced in CHO cells, and purified by affinity chromatography.
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- Ter Muelen J. et al., 2006. Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants. PLos Med. 3(7):e237.
- Yuan M. et al., 2020. A highly conserved cryptic epitope in the receptor-binding domains of SARS-CoV-2 and SARS-CoV. Science. DOI: 10.1126/science.abb7269.
- Tian X. et al., 2020. Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody. Emerging Microbes & Infections. 9(1):382-385.
- Huo J. et al., 2020. Neutralization of SARS-CoV-2 by destruction of the prefusion Spike. bioRxiv. DOI:10.1101/2020.05.05.079202.