Anti-mCTLA-4

InvivoGen Kontakt z doradcą
Antibody against murine CTLA-4
Anti-mCTLA-4

Anti-mCTLA4-mIgG2a InvivoFit™ is a mouse anti-mouse monoclonal antibody (mAb) featuring the variable region of the previously described anti-mCTLA-4 9D9 mAb [1] and a murine IgG2a constant region. The 9D9 mAb has a sequence that is 100% murine (constant and variable regions), ensuring minimal immunogenicity upon repeated injections into mice. This mAb targets murine cytotoxic T-lymphocyte-associated protein 4 (mCTLA-4) and blocks CTLA4-mediated negative signals downregulating T cell activation.  Thus, anti-mCTLA-4 mAb unleashes T cell activity [1, 2].

Notably, the 9D9 mAb, along with the most commercially available anti-mCTLA4 mAbs feature the IgG2b isotype which displays low antitumor activity in vivo [2]. To overcome this issue, Anti-mCTLA4-mIgG2a InvivoFit™ was generated by recombinant technology and features the murine IgG2a isotype, which mediates potent cytotoxic functions. Anti-mCTLA4-mIgG2a is provided in an InvivoFit™ grade, a high-quality standard specifically adapted to in vivo studies.

Key features of Anti-mCTLA4-mIgG2a InvivoFit™:

  • Sequence is 100% murine
  • Features the IgG2a isotype (associated with high antitumor activity)
  • Guaranteed sterile, endotoxin level < 1 EU/mg
  • Specifically designed for in vivo studies in mice
  • Low aggregation < 5%
  • Produced in animal-free facilities and defined media

Anti-mCTLA4-mIgG2a InvivoFit™ is produced in Chinese hamster ovary (CHO) cells, purified by affinity chromatography with protein A, and it's binding to mCTLA-4 is validated using ELISA.

References:

  1. Selby MJ. et al., 2013. Anti-CTLA-4 antibodies of IgG2a isotype enhance antitumor activity through reduction of intratumoral regulatory T cells. Cancer Immunol Res. 1(1):32-42.
  2. Ribas A. & Wolchock J.D., 2018. Cancer immunotherapy using checkpoint blockade. Science. 359:1350-55.

Podobne produkty

Anti-mTIGIT

Anti-mTIGIT

InvivoGen
Więcej
LumiKine™ Xpress hIFN-β 2.0

LumiKine™ Xpress hIFN-β 2.0

InvivoGen
Więcej
Anti-IL-6

Anti-IL-6

InvivoGen
Więcej