Human ACE2 Protein

Protein description

Human ACE2 (angiotensin I-converting enzyme-2) is a type I surface transmembrane protein expressed in arteries, heart, kidneys, and epithelia of the lung and small intestine [1, 2]. ACE2 belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases [2, 3].
ACE2 also plays a critical role in the human pathogenesis of the coronavirus disease-19 (COVID-19) caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2, or 2019-nCoV). Indeed, hACE2 is now established as the receptor for the Spike (S) protein of the SARS-CoV and SARS-CoV-2 coronaviruses, facilitating the viral entry into target cells [4-6]. The blockade of the ACE2 receptor and the delivery of an excessive soluble form of ACE2 are among the investigated strategies to treat COVID-19.
The soluble hACE2-Fc protein was generated by fusing the C-terminus of the human ACE2 extracellular domain [M1-S740] to a human IgG1 Fc region.
This protein has been produced in CHO cells and purified by protein G affinity chromatography (See Details and Specifications for more information).

Applications

• SARS-CoV and SARS-CoV-2 neutralization assays
• Screening of small molecules inhibitors or of neutralizing antibodies able to block Spike-RBD and ACE2 interaction 

Quality control

• Size and purity confirmed by SDS PAGE
• Protein validated by ELISA upon incubation with a coated Spike-RBD-His protein and an Anti-human IgG1-HRP detection antibody

Learn more about SARS-CoV-2 infection cycle, immune responses, and potential therapeutics.

References

1. Harmer D. et al., 2002. Quantitative mRNA expression profiling of ACE2, a novel homolog of angiotensin-converting enzyme. FEBS Letters. 532(1-2):107-110.
2. Hamming I. et al., 2004. Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. J. Pathol. 203:631-637.
3. Donoghue M. et al., 2000. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. Cir. Research. 87(5):e1-e9.
4. Li W. et al., 2003. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 426(6965):450-454.
5. Hoffmann M. et al., 2020. SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell. 181:1-16.
6. Zhou P. et al., 2020. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 579(7798):270-273.