Toll-like receptor 5 (TLR5) is a type-1 transmembrane receptor comprising an N-terminal extracellular leucine rich repeat domain and a C-terminal intracellular TIR signaling domain.
The soluble TLR5 receptor, hTLR5-Fc, was generated by fusing the N-terminal extracellular domain of human TLR5 (aa 21-639) to the N-terminus of an engineered Fc region of human IgG1 with a 2 amino acid linker.
The hTLR5-hFc fusion has an apparent molecular weight of 110 kDa on SDS-PAGE.
Fc-hTLR5 is expressed in CHO cells and purified by protein G affinity chromatography.