B16-Blue™ derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP reporter gene under the control of an inducible ISG54 promoter enhanced by a multimeric ISRE.
Stimulation of B16-Blue™ cells triggers the production of SEAP.
Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.
