HCT116 colorectal carcinoma

InvivoGen Kontakt z doradcą
Human HCT116 colorectal carcinoma - NF-κB & IRF reporter cells
HCT116 colorectal carcinoma

HCT116-Dual™ cells are adherent epithelial cells that have been derived from the human HCT116 colorectal carcinoma cell line by stable integration of two inducible reporter constructs.

HCT116-Dual™ cells  express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-­1 binding sites.

HCT116-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.

As a result, HCT116-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase.

Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™, a Lucia luciferase detection reagent.

Podobne produkty

Jurkat T Lymphocytes

Jurkat T Lymphocytes

InvivoGen
Więcej
HepG2 hepatoma

HepG2 hepatoma

InvivoGen
Więcej
B16 melanoma

B16 melanoma

InvivoGen
Więcej