HepG2 hepatoma

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Human HepG2 liver carcinoma - Transcription factor reporter cells
HepG2 hepatoma

The HepG2 cell line is a human hepatoma commonly used to study the cellular responses upon infection with hepatotropic pathogens or upon activation of the aryl hydrocarbon receptor (AhR) pathways by environmental pollutants or diet-derived compounds.

InvivoGen provides HepG2 cell lines engineered to express the secreted embryonic alkaline phosphatase (SEAP) and Lucia luciferase reporter proteins for assessing the induction of the NF-κB, the interferon regulatory factor (IRF), or the AhR signaling pathways.

  • HepG2-Dual™ cellsare designed to allow the simultaneous study of the NF-κB and IRF pathways by measuring the activity of SEAP and Lucia luciferase, respectively.
  • HepG2-Lucia™ AhR cellsare designed for the study of the AhR genomic signaling pathway by monitoring the Lucia luciferase activity.

SEAP and Lucia luciferase reporter proteins are readily measurable in the cell culture supernatant using QUANTI-Blue™ and QUANTI-Luc™, respectively.

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