HT29-Lucia™ AhR Cells

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Human HT29 colon adenocarcinoma - AhR reporter cells
HT29-Lucia™ AhR Cells

HT29-Lucia™ AhR cells are engineered from the human HT-29 colon adenocarcinoma cell line for the study of the aryl hydrocarbon receptor (AhR) genomic signaling induction (see details), by monitoring the activity of the Lucia luciferase reporter protein.

AhR is a ligand-dependent transcriptional factor widely expressed in barrier tissues. Notably, AhR plays a important role in gut-microbiota and host's immune homeostasis [1].

HT-29 cells are characterized by:

  • Endogenous AhR expression [2]
  • Ability to differentiate into intestinal enterocyte-like cells and mucus-producing cells [3]

Therefore, HT29-Lucia™ AhR cells are highly relevant for studying intestinal microbiota-related ligands for AhR.

HT29-Lucia™ AhR cells express the secreted Lucia luciferase reporter gene under under the control of a minimal promoter coupled with the human Cyp1a1 gene entire regulatory sequence, which contains six dioxin responsive elements (DREs).
As a result, these cells allow to study the AhR genomic signaling pathway, by monitoring the activity of Lucia luciferase in the cell culture supernatant when using the QUANTI-Luc™ or QUANTI-Luc™ Gold detection reagents.

HT29-Lucia™ AhR cells are resistant to Zeocin™.

References

  1. Lamas B et al.< 2018. Aryl hydrocarbon receptor and intestinal immunity. Mucosal Immunol. 11:1024-38.
  2. Park JH. et al., 2018. Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. Oncol Rep. 39(4):1930-8.
  3. artínez-Maqueda D. et al. 2015. HT29 Cell Line. Bioactives on Health: in vitro and ex vivo models. Springer. Chapt 1.

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