pBROAD3 plasmids were designed for the expression of your favorite gene in virtually all tissues of new transgenic mice and rats. pBROAD3 plasmids feature the ubiquitous mouse ROSA26 promoter, a high CpG content and TATA-less promoter. It directs in vivo expression of the transgene in most tissue types including cells of the developing germ line and hematopoietic lineage. It is also very effective in vitro in a very broad range of mammalian cell lines.