InvivoGen provides two different E. coli competent strains:
- GT115 cells are specifically designed for cloning and propagation of plasmids containing hairpin structures and the R6K gamma origin of replication, such as pCpG-siRNA plasmids
- GT116 cells are specifically designed for cloning and propagation of shRNA-expressing plasmids which contain hairpin structures, such as psiRNA plasmids.
The strains are made chemically competent by an optimized procedure followed by transformation efficiency verification and are provided frozen (ChemiComp).
