ULTRATOOLS DNA Polymerase 5U/µl
Biotools Kontakt z doradcąUltratools DNA polymerase is a recombinant DNA Polymerase from Thermus s.p. and cloned in E. coli, which undergoes an additional nonchromatographic method of purification.
The ultrapure enzyme is free of unspecific endo- or exonuclease activities.
Highly thermostable and processive enzyme suitable for applications including:
- Amplification of complex secondary structures
- Amplifications involving bacterial DNA sequences homologous to E. coli
- Amplification of PCR products of up to 5 kb
- Colony screening
- Recombinant cDNA construction
- Genotyping
Contents:
Ultratools DNA Polymerase is supplied with the choice of 10 X Standard Reaction Buffer with 2 mM MgCl2 or a reaction buffer without MgCl2 (10 X Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.
Specifications:
- Polymerase: ULTRATOOLS
- Hot Start effect: No
- Fidelity (vs Taq): ++
- Concentration: 5 U/μl
- GC-Rich PCR Performance: No
- Reaction Format: Separate components
- Amplification size: Up to 5 kb
- Product size: 100 U, 250 U
- Exonuclease Activity: 5’ - 3’
- Shipping condition: Cold shipping
- Storage condition: -20ºC
- Regulatory Statement: For research use only. Not for use in diagnostic procedures
