Pfu DNA polymerase 5U/µl

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High-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3'-5' exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases).
Pfu DNA polymerase 5U/µl

High-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3'-5' exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases). 

Biotools Pfu DNA Polymerase generates blunt-ended amplification products, enabling their cloning into blunt-ended vectors. 

It is suitable for applications including:

  • Cloning 
  • Mutagenesis
  • Achieving greater fidelity than with Taq DNA polymerase
  • Amplifying fragments of up to 5 kb

Contents:

Biotools Pfu DNA Polymerase is supplied with the choice of 10 X Standard Reaction Buffer including 2 mM MgCl2 or a reaction buffer without MgCl2 (10 X Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.

Specifications:

  • Polymerase: Pfu
  • Hot Start effect: No
  • Fidelity (vs Taq): ++++
  • Concentration: 5 U/μl
  • GC-Rich PCR Performance: No
  • Reaction Format: Separate Components
  • Amplification size: Up to 5 kb
  • Product size: 100 U, 250 U
  • Exonuclease Activity: 3’ - 5’
  • Shipping condition: Cold shipping
  • Storage condition: -20ºC
  • Regulatory Statement: For research use only. Not for use in diagnostic procedures

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