DNA Polymerase 5U/µl

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Recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5'-3' polymerase and 5'-3' exonuclease activities and a reduced error rate (1 - 10 x 10-6).
DNA Polymerase 5U/µl

Thermostable enzyme perfect for routine amplifications including:

  • Amplification of PCR products of up to 5
  • Colony screening 
  • Recombinant cDNA construction
  • Genotyping

Contents:

Biotools DNA Polymerase (1U/µl and 5U/µl) is supplied with the choice of 10 X Standard Reaction Buffer with 2 mM MgCl2 or a reaction buffer without MgCl2 (10 X Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.

Specifications:

  • Polymerase: BIOTOOLS DNA Polymerase
  • Hot Start effect: No
  • Fidelity (vs Taq): ++
  • Concentration: 5 U/μl
  • GC-Rich PCR Performance: No
  • Reaction Format: Master Mix
  • Amplification size: Up to 5 kb
  • Product size: 500 U, 1.000 U, 5.000 U, 10.000
  • Exonuclease Activity: 5’ - 3’
  • Shipping condition: Cold shipping
  • Storage condition: -20ºC
  • Regulatory Statement: For research use only. Not for use in diagnostic procedures

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