CERTAMP Kit for COMPLEX AMPLIFICATIONS

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Certamp kits include an exclusive combination of proof-reading and non-proofreading DNA Polymerases, together with cofactors and an enhancing buffer to increase the overall yield of long and/or difficult amplification reactions.
CERTAMP Kit for COMPLEX AMPLIFICATIONS

The enzyme mix maintains maximum efficiency while keeping the lowest error rate, as the low processivity of proofreading enzymes is compensed by the high processivity of non-proofreading enzymes.

Certamp kit for Complex Amplifications is suitable for:

  • Amplification of GC rich templates (of up to 8 kb)
  • Amplification of templates with secondary structures 
  • Amplification of DNA for cloning

Contents:

Certamp kit for Complex Amplifications is provided with Certamp Enzyme Mix, buffers (10X Reaction Buffer MgCl2 Free and 10X Certamp Complex Buffer MgCl2 Free), and 50 mM MgCl2.

Specifications:

  • Polymerase: Certamp kit for Complex Amplifications
  • Hot Start effect: No
  • Fidelity (vs Taq): +++
  • Concentration: 1 U/μl
  • GC-Rich PCR Performance: Yes
  • Reaction Format: Master Mix
  • Amplification size: Up to 8 kb
  • Product size: 250 rxns, 500 rxns
  • Exonuclease Activity: 5’ - 3’ and 3’ - 5’
  • Shipping condition: Cold shipping

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