CERTAMP Kit for COMPLEX AMPLIFICATIONS
Biotools Kontakt z doradcąCertamp kits include an exclusive combination of proof-reading and non-proofreading DNA Polymerases, together with cofactors and an enhancing buffer to increase the overall yield of long and/or difficult amplification reactions.
The enzyme mix maintains maximum efficiency while keeping the lowest error rate, as the low processivity of proofreading enzymes is compensed by the high processivity of non-proofreading enzymes.
Certamp kit for Complex Amplifications is suitable for:
- Amplification of GC rich templates (of up to 8 kb)
- Amplification of templates with secondary structures
- Amplification of DNA for cloning
Contents:
Certamp kit for Complex Amplifications is provided with Certamp Enzyme Mix, buffers (10X Reaction Buffer MgCl2 Free and 10X Certamp Complex Buffer MgCl2 Free), and 50 mM MgCl2.
Specifications:
- Polymerase: Certamp kit for Complex Amplifications
- Hot Start effect: No
- Fidelity (vs Taq): +++
- Concentration: 1 U/μl
- GC-Rich PCR Performance: Yes
- Reaction Format: Master Mix
- Amplification size: Up to 8 kb
- Product size: 250 rxns, 500 rxns
- Exonuclease Activity: 5’ - 3’ and 3’ - 5’
- Shipping condition: Cold shipping
