CERTAMP Kit for LONG AMPLIFICATIONS

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Certamp kits for Long Amplifications include an exclusive combination of proof-reading and non-proofreading DNA Polymerases, together with cofactors and an enhancing buffer to increase the overall yield of long and/or difficult amplification reactions.
CERTAMP Kit for LONG AMPLIFICATIONS

The enzyme mix maintains maximum efficiency while keeping the lowest error rate, as the low processivity of proofreading enzymes is compensated by the high processivity of non-proofreading enzymes.

Certamp kit for Long Amplifications is suitable for: 

  • Amplification of DNA fragments of up to 40 kb from λ fage or similar DNAs
  • Amplification of DNA fragments of up to 15 kb from genomic DNA 

Contents:

Certamp kit for Long Amplifications is provided with Certamp Enzyme Mix, two buffers (10X Reaction Buffer MgCl2 Free and 10X Certamp Long Buffer MgCl2 Free)and 50 mM MgCl2.

Specifications:

  • Polymerase: Certamp kit for Long Amplifications
  • Hot Start effect: No
  • Fidelity (vs Taq): +++
  • Concentration: 1 U/μl
  • GC-Rich PCR Performance: No
  • Reaction Format: Master Mix
  • Amplification size:
    Up to 15 kb
    Up to 40 kb
  • Product size: 250 rxns, 500 rxns
  • Exonuclease Activity: 5’ - 3’ and 3’ - 5’
  • Shipping condition: Cold shipping
  • Storage condition: -20ºC
  • Regulatory Statement: For research use only. Not for use in diagnostic procedures

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