CERTAMP Kit for LONG AMPLIFICATIONS
Biotools Kontakt z doradcąCertamp kits for Long Amplifications include an exclusive combination of proof-reading and non-proofreading DNA Polymerases, together with cofactors and an enhancing buffer to increase the overall yield of long and/or difficult amplification reactions.
The enzyme mix maintains maximum efficiency while keeping the lowest error rate, as the low processivity of proofreading enzymes is compensated by the high processivity of non-proofreading enzymes.
Certamp kit for Long Amplifications is suitable for:
- Amplification of DNA fragments of up to 40 kb from λ fage or similar DNAs
- Amplification of DNA fragments of up to 15 kb from genomic DNA
Contents:
Certamp kit for Long Amplifications is provided with Certamp Enzyme Mix, two buffers (10X Reaction Buffer MgCl2 Free and 10X Certamp Long Buffer MgCl2 Free)and 50 mM MgCl2.
Specifications:
- Polymerase: Certamp kit for Long Amplifications
- Hot Start effect: No
- Fidelity (vs Taq): +++
- Concentration: 1 U/μl
- GC-Rich PCR Performance: No
- Reaction Format: Master Mix
- Amplification size:
Up to 15 kb
Up to 40 kb - Product size: 250 rxns, 500 rxns
- Exonuclease Activity: 5’ - 3’ and 3’ - 5’
- Shipping condition: Cold shipping
- Storage condition: -20ºC
- Regulatory Statement: For research use only. Not for use in diagnostic procedures
