DNA Amplitools Fast Master Mix

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The new developed DNA AmpliTools Fast Master Mix (2X) is a sensitive, premix solution for performing rapid cycling protocols of a wide range of templates (including genomic DNAs and templates rich in CG-content).
DNA Amplitools Fast Master Mix

It has been designed to reduce PCR reaction time to as little as 25 min for 500 bp fragments.

The DNA AmpliTools Fast Master Mix has been developed by Biotools to provide fast DNA amplifications with a shortened PCR cycling time on all thermal cyclers. The combination of Biotools HotSplit DNA Polymerase (active at high temperatures) and the new Fast Reaction Buffer in a convenient master mix format minimises nonspecific amplification products, primer-dimers, and background while significantly reducing PCR duration. This mix is recommended for amplifications up to 8 kb inlength.

It is a ready-to-use, 2X-concentrated, hot start master mix that reduces set up time, liquid handling steps and prevents cross-contamination. With the exception of primers and template, the DNA AmpliTools Fast Master Mix provides all the necessary reagents at optimal concentrations to perform PCRs. PCR setup is quick and convenient as all reaction components can be combined at room temperature.

The DNA AmpliTools Fast Master Mix makes highly specific hot start PCR extremely fast and simple, with minimal optimisation required. The pre-optimised PCR protocol avoids the need for redesign of primers for established assays.

The DNA AmpliTools Master Mixes of Biotools allow for convenient and easy to use PCRs, and they are ideally suited to routine PCR applications.

Applications of DNA AmpliTools Fast Master Mix:

  • Fast PCR (<25 min-500 bp)
  • High specificity PCR (up to 8 kb)
  • Primer extension
  • Generation of PCR products for TA cloning
  • Gene sequencing
  • RT-PCR

Note: This master mix is not recommended for certain experiments dealing with amplification of
sequences homologous to those found in E. coli.

Components: 

The DNA AmpliTools Fast Faster Mix (2X) contains recombinant Biotools HotSplit DNA Polymerase; dNTPs; MgCl2; the new Fast Reaction Buffer; and stabilisers at optimal concentrations for efficient and fast amplification protocols.

Specifications:

  • Master Mix: DNA AmpliTools Fast Master Mix
  • Hot Start effect: No
  • GC-Rich PCR Performance: No
  • Reaction Format: Liquid format
  • Amplification size: Up to 8 kb
  • Product size: 100 rxns, 200 rxns
  • Shipping condition: Cold shipping
  • Storage condition: -20ºC
  • Regulatory Statement: For research use only. Not for use in diagnostic procedures

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