DNA AmpliTools Multiplex Master Mix

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The new developed DNA AmpliTools Multiplex Master Mix (2X) is a sensitive, premix solution for performing simultaneous amplification of multiple targets using multiple primers.
DNA AmpliTools Multiplex Master Mix

It has been designed to reduce PCR reaction time and to enable an efficient use of valuable samples.

The DNA AmpliTools Multiplex Master Mix has been developed by Biotools including Biotools HotSplit DNA Polymerase and the new Reaction Buffer in a convenient master mix format that minimises nonspecific amplification products and primer-dimers. This mix is recommended for amplifications up to 7 kb in length.

The ready to use format dramatically reduces set up time and liquid handling steps, thereby minimising the risk of contamination. With the exception of primers and template, the convenient DNA AmpliTools Multiplex Master Mix (2X) provides all the necessary reagents at optimal concentrations to perform multiplex PCRs without previous optimization.  

The DNA AmpliTools Master Mixes of Biotools allow convenient and easy to use PCRs, and they are ideally suited for routine PCR applications.

Applications of DNA AmpliTools Multiplex Master Mix:

  • Amplification of multiple fragments for SNPs genotyping
  • High specificity PCR (up to 7 kb)
  • Typing of transgenic organism.
  • Generation of PCR products for cloning
  • Detection of pathogen

Note: This master mix is not recommended for certain experiments dealing with amplification of sequences homologous to those found in E. coli.

Components: 

The DNA AmpliTools Multiplex Master Mix contains Biotools HotSplit DNA Polymerase, dNTPs, 3.0 mM MgCl2, and Reaction Buffer optimised to carry out multiplex amplification reactions. 

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