DNA AmpliTools Green Master Mix

The DNA AmpliTools Green Master Mix includes a density reagent and two tracking dyes that allow direct loading onto a gel and monitoring of progress during electrophoresis. 

The ready to use format dramatically reduces set up time, liquid handling steps, thereby minimising the risk of contamination. With the exception of primers and template, the convenient DNA AmpliTools Green Master Mix provides all the necessary reagents at optimal concentrations to perform PCRs for targets up to 10 kb in length. The DNA AmpliTools Green Master Mix (2X) works with different reaction volumes between 20-50 μl. 

The DNA AmpliTools Green Master Mixes of Biotools allow for convenient and easy to use PCRs, and they are ideally suited to routine PCR applications with minimal optimisation. 

Applications: 

• Routine PCR (up to 10 kb) 
• High throughput PCR 
• Primer extension 
• Generation of PCR products for TA cloning 
• Gene sequencing 
• RT-PCR 

Note: This master mix is not recommended for certain experiments dealing with amplification of sequences homologous to those found in E. coli. 

Components: The DNA AmpliTools Green Master Mix (2X) contains recombinant Biotools DNA Polymerase; dNTPs; MgCl₂; Reaction Buffer; stabilisers; and a proprietary buffer containing a compound that increases sample density, and two dyes, which function as loading dyes when reaction products are analised by agarose gel electrophoresis. The blue dye migrates with 3-5 kb DNA fragments, and the orange dye migrates faster than 100 bp DNA fragments in 1% agarose gel.