DNA Amplitools Complex Master Mix

Genomic DNA, GC-rich templates or those with a secondary structure are difficult to amplify. The main reason for this difficulty is that conventional polymerases introduce errors during DNA replication that can stop the amplification reaction, thereby resulting in truncated products and/or error accumulation in the amplified sequence and compromising the yield and fidelity of the PCR. 

The DNA AmpliTools Complex Master Mix overcomes these difficulties by incorporating a mix of highly efficient enzymes, including Biotools HotSplit DNA Polymerase, which has a hot start effect, and Biotools Pfu DNA Polymerase, which has proofreading activity. The combination of these enzymes, together with the special reaction buffer and cofactors included in the mix, results in high yield and copy fidelity in complex amplification reactions. The hot start nature of the HotSplit DNA Polymerase contributes significantly to the specificity achieved with the DNA AmpliTools Complex Master Mix by reducing the formation of primer dimers and nonspecific amplification products. 

Applications of DNA AmpliTools Complex Master Mix: 

• Amplification of GC-rich DNA templates 
• Amplification of DNA templates rich in secondary structures 
• Amplifications using tiny amount of template (detection limit of 0.1 pg) 
• Amplification of fragments up to 10 kb (genomic DNA) 
• Amplification of fragments up to 30 kb (lambda DNA) 

Components of DNA AmpliTools Complex Master Mix:

Biotools HotSplit DNA Polymerase; Biotools Pfu DNA Polymerase; dNTPs: MgCl2; reaction buffer; adjuvants and stabilisers at concentrations suitable for performing a wide range of DNA amplification reactions.