DNA AmpliGel Master Mix-Plates

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Biotools has developed a new PCR product using one of its international patents: GELIFICATION.
DNA AmpliGel Master Mix-Plates

The process is simple because all the reagents needed for the PCR reaction are included in each vial, so just add the sample (DNA and primers). 

Features:

READY-TO-USE 

  • One well- One reaction format
  • Ready for in-field manipulation
  • No optimization needed

LESS MANIPULATION & LESS CONTAMINATION

  • Only need to add primers and DNA sample.
  • Customized plates available: Give us your primers and we will include them in the Gelified reaction.

SHIPMENT AT ROOM TEMPERATURE AND STORAGE AT 4ºC

  • Stable for long time periods at 4ºC.

HOT-START EFFECT

  • Reaction starts when the Gel is completely rehydrated with the increase of temperature.

NO NEED TO RESUSPEND

  • Reaction will become liquid automatically with the increase of temperature (no handling).

ADVANTAGES vs LYOPHILIZATION

  • Components keep their activity.
  • No need to resuspend. 
  • Final concentration is mantained.


DNA AmpliGel Master Mix - Plates and the gelified mix reagents can be stored at 4 °C respectively for long periods of time. The Ready-to-Use format minimises risk of contaminations, while promoting rapid sample processing ideal for routine applications. 

This enzyme is a recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5'-3' polymerase and 5'-3' exonuclease activities, and reduced error rates (1 - 10x 10-6). 

Thermostable enzyme perfect for routine amplifications including:

  • Amplification of PCR products of up to 5 Kb 
  • Colony screening 
  • Recombinant cDNA construction 
  • Genotyping 
  • Routine DNA amplifications 


DNA AmpliGel Master Mix - Plates is available in Vials (fv 25 µl) and in 96 well Plates (fv 25 µl). 

Contents:

DNA AmpliGel Master Mix - Plates 96 well Plates is supplied with 10 X Standard Reaction Buffer including 2 mM MgC2 and dNTPs. 

DNA AmpliGel Master Mix - Plates Vials (0,2 vials) 96 vials. Each vial includes Standard Reaction Buffer with 2 mM MgCl2, dNTPs, and DNA polymerase.

Specifications:

  • Master Mix: DNA AmpliGel Master Mix - Plates
  • Hot Start effect: Yes
  • GC-Rich PCR Performance: No
  • Reaction Format: Gelified format and Ready to use
  • Amplification size: Up to 5 kb
  • Product size: 10 plates, 20 plates
  • Shipping condition: Room temperature
  • Storage condition: 4ºC
  • Regulatory Statement: For research use only. Not for use in diagnostic procedures

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