Master Mix (High SQEP Master Mix)

The high specificity and sensitivity of this Master Mix is achieved by the use of the new Biotools High Retrotrascriptase and the Biotools HotSplit DNA Polymerase, together with a specialised buffer, in an easy-to-handle format

The Master Mix has been designed to deliver maximum efficiency, precision and sensitivity of quantitative RT-PCR. For these purpose, two high-performance enzymes, a thermostable reverse transcriptase and a DNA polymerase with hot start activity, carry out reactions. Both cDNA synthesis and PCR are performed sequentially in a single tube due to a single buffer system formulated to ensure specific primer annealing.

High Scriptools-Quantimix Easy Probes Master Mix provides a convenient and fast procedure, one step only, for synthesizing cDNA and DNA amplification in real time. All reagents necessary for both reactions are added in one tube at the same time which confers an easy-to-handle format saving manipulation time and reducing contamination risks without compromising the efficiency or sensitivity of the kit..

The Master Mix has been optimised with RNA from different origins including RNA virus (e.g. RNA from HCV; HIV; and H1N1 virus, among others).