High Scriptools-Quantimix Easy Master Mix (High SQE Master Mix)

This one-step, two-enzyme Master Mix uses Biotools High Retrotrascriptase and Biotools HotSplit DNA Polymerase for detection of any type of RNA sample.  

The Master Mix has been designed to deliver maximum efficiency, precision and sensitivity of quantitative RT-PCR with intercalating fluorophores. For these purpose two high-performance enzymes, a thermostable reverse transcriptase and a DNA polymerase with hot start activity, carry out reactions. Both cDNA synthesis and PCR are performed sequentially in a single tube due to a single buffer system formulated to ensure specific primer annealing

The real time detection of amplified products is performed by monitoring the increase of fluorescence after each amplification cycle. The fluorescent signal is generated by the incorporation of intercalating fluorophores.

The High Scriptools-Quantimix Easy Master Mix provides a convenient and fast procedure, one step only, for synthesizing cDNA and DNA amplification in real time. All reagents necessary for both reactions are added in one tube at the same time which confers a rapid and easy-to-handle format saving manipulation time and reducing contamination risks without compromising the efficiency or sensitivity of the kit.