HIGH RNase INHIBITOR
Biotools Kontakt z doradcąHigh RNase Inhibitor inhibits, by binding in a potent non-competitive inhibitor of a wide spectrum of RNases, including RNase A, RNase B and RNase C.
It is a recombinant mammalian protein, manufactures in E. coli, with a monomeric structure of 49.6 kDa.
Applications
The inhibitor protects RNA in the following applications:
- First strand cDNA synthesis
- RNA amplification
- RNA purification and storage
- In vitro transcription
- In vitro translation
- Separation and identification of specific ribonuclease activities
- Removal of gDNA from RNA preparations
- Synthesis of RNA probes
- Synthesis of cDNA probes Storage conditions High RNase Inhibitor can be store at - 20°C in a constant temperature freezer through the expiration date printed on the label. Avoid exposure to frequent temperature changes. For frequent use divide the product in aliquots to avoid multiple freeze-thaw cycles.
Activity Unit
One unit of the High RNase Inhibitor inhibits 5 ng of RNase A by 50%.
Activation-inactivation
The High RNase Inhibitor protects RNA from degradation at temperatures ≤ 55 °C.
This product is inactivated by heating at 75 °C for 10 minutes. Common denaturants and oxidizing (eg. SDS, urea) strongly inhibit the High RNase Inhibitor and release the RNase bound.
