HIGH RNase INHIBITOR

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High RNase Inhibitor inhibits, by binding in a potent non-competitive inhibitor of a wide spectrum of RNases, including RNase A, RNase B and RNase C.
HIGH RNase INHIBITOR

It is a recombinant mammalian protein, manufactures in E. coli, with a monomeric structure of 49.6 kDa.

Applications

The inhibitor protects RNA in the following applications:

  • First strand cDNA synthesis
  • RNA amplification
  • RNA purification and storage
  • In vitro transcription
  • In vitro translation
  • Separation and identification of specific ribonuclease activities
  • Removal of gDNA from RNA preparations
  • Synthesis of RNA probes
  • Synthesis of cDNA probes 
Storage conditions 
High RNase Inhibitor can be store at - 20°C in a constant temperature freezer through the expiration date printed on the label. 
Avoid exposure to frequent temperature changes. For frequent use divide the product in aliquots to avoid multiple freeze-thaw cycles.

Activity Unit

One unit of the High RNase Inhibitor inhibits 5 ng of RNase A by 50%.

Activation-inactivation

The High RNase Inhibitor protects RNA from degradation at temperatures ≤ 55 °C.

This product is inactivated by heating at 75 °C for 10 minutes. Common denaturants and oxidizing (eg. SDS, urea) strongly inhibit the High RNase Inhibitor and release the RNase bound.

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