CD19 Fab-Strep, human

IBA Lifescience Kontakt z doradcą
Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for human CD19
CD19 Fab-Strep, human

CD19 Fab-Strep is a recombinantly expressed low affinity Fab fragment fused to a Twin-Strep-tag®. Fab-Streps were developed for Traceless Affinity Cell Selection (Fab-TACS®), which is based on our Strep-tag® technology. Due to their low affinity for the target, an increase in avidity is required to ensure sufficient binding to the cell surface. Combining Fab-Streps with a Strep-Tactin® backbone leads to the formation of complexes that stably bind to cells.

Depending on the Strep-Tactin® backbone, the following applications are possible:

  • Fluorescent cell staining & sorting: Fab-Streps are combined with fluorophore-conjugated Strep-Tactin®.
  • Affinity chromatographic cell isolation: Fab-Streps are loaded in Strep-Tactin® TACS Agarose columns.

By addition of biotin, the labeling reagents dissociate mildly and rapidly from the selected target cells in all applications, yielding minimally manipulated cells for unbiased further use.

Specifications

  • Endotoxin Level: ≤ 0.1 EU/µg by LAL test
  • Form: Lyophilized
  • Possible Application: Fluorescent cell staining & sorting, affinity chromatographic cell isolation
  • Purity: ≥ 90%
  • Reconstitution: Reconstitute in 1 ml buffer, e.g. 1x PBS containing 1 mM EDTA and 0.5% BSA, for a final concentration of 50 µg/ml.
  • Specificity: Human CD19

Podobne produkty

MHC I-Strep HLA-B*0702; CMV pp65 (RPHERNGFTVL)

MHC I-Strep HLA-B*0702; CMV pp65 (RPHERNGFTVL)

IBA Lifescience
Więcej
MHC I-Strep HLA-A*0201; Survivin (TLPPAWQPFL)

MHC I-Strep HLA-A*0201; Survivin (TLPPAWQPFL)

IBA Lifescience
Więcej
HsPSMA peptide SAVKNFTEI (H-2 Db)

HsPSMA peptide SAVKNFTEI (H-2 Db)

IBA Lifescience
Więcej