CD19 Nano-TACS® Agarose Column Starter Kit, mouse

Nano-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD19 Nano-TACS® Agarose Column Starter Kit allows label-free isolation of murine CD19+ B cells from splenocytes or other cell suspensions. CD19 is an adaptor protein that is involved in signaling pathways.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity nanobodies fused to a Twin-Strep-tag® (Nano-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Nano-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Nano-Streps. After elution, the Nano-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:

• Highly pure cells
• Reversible binding to the target
• Highly viable, non-activated cells
• Label-free cells due to low affinity nanobodies

The kit contains:

• Strep-Tactin® TACS Agarose columns, 0.3 ml(2x)
• CD19 Nano-Strep, mouse
• 10x Buffer CI
• Biotin stock solution
• TACS Column Adapter