CD31 Fab-TACS® Agarose Column Starter Kit, human

Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD31 Fab-TACS® Agarose Column Starter Kit allows label-free isolation of human CD31+ cells from whole blood, buffy coat, PBMCs or other cell suspensions. CD31 is also known as platelet endothelial cell adhesion molecule (PECAM-1) and expressed on different cell types including endothelial cells, platelets, monocytes, neutrophils and lymphocytes.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their reduced affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:

• Highly pure cells
• Reversible binding to the target
• Highly viable, non-activated cells
• Label-free cells due to low affinity Fab fragments

The kit contains:

• Strep-Tactin® TACS Agarose columns, 1 ml (2x)
• CD31 Fab-Strep, human
• 10x Buffer CI
• Biotin stock solution
• TACS Column Adapter
• Flow Restrictor, pack of 5