CD4 Fab-Strep, mouse

IBA Lifescience Kontakt z doradcą
Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for murine CD4
CD4 Fab-Strep, mouse

CD4 Fab-Strep is a recombinantly expressed low affinity Fab fragment fused to a Twin-Strep-tag®. Fab-Streps were developed for Traceless Affinity Cell Selection (Fab-TACS®), which is based on our Strep-tag® technology. Due to their low affinity for the target, an increase in avidity is required to ensure sufficient binding to the cell surface. Combining Fab-Streps with a Strep-Tactin® backbone leads to the formation of complexes that stably bind to cells.

Depending on the Strep-Tactin® backbone, the following applications are possible:

  • Fluorescent cell staining & sorting: Fab-Streps are combined with fluorophore-conjugated Strep-Tactin®.
  • Affinity chromatographic cell isolation: Fab-Streps are loaded in Strep-Tactin® TACS Agarose columns.

By addition of biotin, the labeling reagents dissociate mildly and rapidly from the selected target cells in all applications, yielding minimally manipulated cells for unbiased further use.

Specifications

  • Endotoxin Level: ≤ 0.1 EU/µg by LAL test
  • Form: Lyophilized
  • Possible Application: Fluorescent cell staining & sorting, affinity chromatographic cell isolation
  • Purity: ≥ 90%
  • Reconstitution: Reconstitute in 1 ml buffer, e.g. 1x PBS containing 1 mM EDTA and 0.5% BSA, for a final concentration of 50 µg/ml.
  • Specificity: Murine CD4

Podobne produkty

MHC I-Strep HLA-A*0201; MART-1 (ELAGIGILTV)

MHC I-Strep HLA-A*0201; MART-1 (ELAGIGILTV)

IBA Lifescience
Więcej
Survivin peptide TLPPAWQPFL (HLA-A*0201)

Survivin peptide TLPPAWQPFL (HLA-A*0201)

IBA Lifescience
Więcej
MHC I-Strep HLA-A*0201; CMV IE-1 (VLEETSVML)

MHC I-Strep HLA-A*0201; CMV IE-1 (VLEETSVML)

IBA Lifescience
Więcej