CD4/CD45RA Fab-TACS® Magnetic Microbead Starter Kit, human

Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD4/CD45RA Fab-TACS® Magnetic Microbead Starter Kit allows label-free isolation of naïve CD4+ T cells (CD4+CD45RA+) from peripheral blood mononuclear cells (PBMCs). CD45RA is an isoform of tyrosine phosphatase CD45 (leukocyte common antigen) that is expressed on different leukocytes including naïve and resting T cells, B cells as well as monocytes.

Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) are incubated with Strep-Tactin® Magnetic Microbeads to form a complex that stably binds to the cell surface. After specific labeling, target cells are bound to a magnet and non-target cells efficiently washed away. In a final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity. After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications.

Advantages:

• High purity of > 90%
• Scalable reagents
• Label-free cells due to low affinity Fab fragments
• Non-activated cells

The kit contains:

• Strep-Tactin® Magnetic Microbeads
• CD4 Fab-Strep, human
• CD45RA Fab-Strep, human
• 10x Buffer CI
• Biotin stock solution